Advanced Fluorescence Fluctuation Spectroscopy with Pulsed Interleaved Excitation
نویسنده
چکیده
Diese Dissertation wurde selbständig, ohne unerlaubte Hilfe erarbeitet.
منابع مشابه
Pulsed interleaved excitation fluctuation imaging.
Fluorescence fluctuation imaging is a powerful means to investigate dynamics, interactions, and stoichiometry of proteins inside living cells. Pulsed interleaved excitation (PIE) is the method of nanosecond alternating excitation with time-resolved detection and allows accurate, independent, and quasi-simultaneous determination of fluorescence intensities and lifetimes of different fluorophores...
متن کاملTime-Resolved Fluorescence Spectroscopy Measures Clustering and Mobility of a G Protein-Coupled Receptor Opsin in Live Cell Membranes
Determining membrane protein quaternary structure is extremely challenging, especially in live cell membranes. We measured the oligomerization of opsin, a prototypical G protein-coupled receptor with pulsed-interleaved excitation fluorescence cross-correlation spectroscopy (PIE-FCCS). Individual cell measurements revealed that opsin is predominantly organized into dimeric clusters. At low conce...
متن کاملCombined Unfolded Principal Component Analysis and Artificial Neural Network for Determination of Ibuprofen in Human Serum by Three-Dimensional Excitation–Emission Matrix Fluorescence Spectroscopy
This study describes a simple and rapid approach of monitoring ibuprofen (IBP). Unfolded principal component analysis-artificial neural network (UPCA-ANN) and excitation-emission spectra resulted from spectrofluorimetry method were combined to develop new model in the determination of IBF in human serum samples. Fluorescence landscapes with excitation wavelengths from 235 to 265 nm and emission...
متن کاملSynthesize and Optical properties of ZnO: Eu Microspheres Based Nano-sheets at Direct and Indirect Excitation
Europium (Eu) doped ZnO microsphere based nano-sheets were synthesized through hydrothermal method. Effects of different concentrations of Europium on structural and optical properties of ZnO nano-sheets were investigated in detail. Prepared un-doped and Eu-doped ZnO samples were characterized using X-Ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX), scanning electron micro...
متن کاملEvaluation of a femtosecond fiber laser for two-photon fluorescence correlation spectroscopy.
This work evaluates a femtosecond fiber laser for use in two-photon fluorescence fluctuation spectroscopy. Fiber lasers present an attractive alternative to Ti:Sapphire systems because of their compact size and portability. Autocorrelation of the second harmonic generation signal from the laser demonstrates that its stability is sufficient for two-photon fluorescence correlation spectroscopy. F...
متن کامل